Preceding her husband on the dais at a rally in Melbourne, Fla., the First Lady started her remarks with the words 'let us pray,' before beginning her recitation of the well-known Christian prayer.
She also briefly addressed her intention to remain true to herself and committed to furthering women and children's issues.
Last week, Mrs. Trump publicly referenced her religion when she tweeted a photo of Mar-a-Lago saying: 'Proud to share part of my family history with Mrs. Abe @BBTSchurch where @POTUS & I were married, where we celebrate and we pray.'
After the prayer, which was followed by applause, she launched into an uncharacteristically strident defense of her husband -- and her own brief tenure as First Lady, reports Daily Mail.
"The America we envision is one that works for all Americans and where all Americans can work and succeed," she said.
"A nation committed to a greater civility and unity between people from all sides of the political divide. I will always stay true to myself and be truthful to you, no matter what the opposition is saying about me," the First Lady said, wearing an Alexander McQueen red frock with a fitted waist and flared skirt that cost $2,200 and $600 Louboutin pumps.
"I will act in the best interests of all of you. I'm committed to creating and supporting initiatives dear to my heart. Which will have impact on women, and children all around the world," she said.
"My husband is creating a country of great safety, and prosperity," she said before introducing President Donald Trump. He delivered a speech, lasting about 45 minutes, in what was billed by the White House as "a campaign rally for America."